|
KMID : 0545120160260030498
|
|
Journal of Microbiology and Biotechnology
2016 Volume.26 No. 3 p.498 ~ p.502
|
|
|
Improving 3’-Hydroxygenistein Production in Recombinant Pichia pastoris Using Periodic Hydrogen Peroxide-Shocking Strategy
|
|
Wang Tzi Yuan
Tsai Yi Hsuan
Yu I Zen
Chang Te Sheng
|
|
|
Abstract
|
|
|
|
3’-Hydroxygenistein can be obtained from the biotransformation of genistein by the engineered Pichia pastoris X-33 strain, which harbors a fusion gene composed of CYP57B3 from Aspergillus oryzae and a cytochrome P450 oxidoreductase gene (sCPR) from Saccharomyces cerevisiae. P. pastoris X-33 mutants with higher 3’-hydroxygenistein production were selected using a periodic hydrogen peroxide-shocking strategy. One mutant (P2-D14-5) produced 23.0 mg/l of 3’-hydroxygenistein, representing 1.87-fold more than that produced by the recombinant X-33. When using a 5 L fermenter, the P2-D14-5 mutant produced 20.3 mg/l of 3’- hydroxygenistein, indicating a high potential for industrial-scale 3’-hydroxygenistein production.
|
|
|
KEYWORD
|
|
|
3’-Hydroxygenistein, orobol, CYP57B3, Pichia pastoris
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|